PMC

Data are the mean ± S.E. L/M ratios (A) or lordosis quality (B) for rats that did or did not receive a prior mating experience (Pretest vs No Pretest) and rats that did or did not experience 10 min of no-contact time in the arena (with male vs without male). Data are for a 10 min test 40 min after injection with 15 mg/kg fluoxetine. Ns for Pretest (male/ no male) and No Pretest (male/ no male), respectively, were 6, 8, 11, and 8. One rat had no mounts and 2 rats had L/M ratios of zero during the pretest and were therefore excluded from the analyses. There was a significant effect of pretest (p < 0.05) but not arena (p > 0.05) and there was no interaction between pretest and arena. Nevertheless, the effect of the pretest on L/M ratios was more robust for rats tested without arena experience while, for lordosis quality, effects were greater in rats with arena experience (Tukey's, q < 0.05). * indicates a significant main effect of the pretest condition. @ indicates significant post hoc comparisons between pretest and no pretest within the same testing area.

Schematic representation of the experimental procedures and groups. (A) the forced swimming test (FST) process consists of two sessions: a pretest swim (pTS, 15 min) and a test swim (5 min) 24 h later. (B) one set of rats was injected with 0.9% saline (SAL, 1 ml/kg, i.p.), desipramine (DMI, 15 mg/kg/ml, i.p.), or citalopram (CIT, 10 mg/kg/ml, i.p.) according to the antidepressant treatments schedule (0.5 h, 19 h, and 23 h after the pretest swim), but not exposed to the pretest swim. (C) another set of rats was treated with saline, desipramine or citalopram after the pretest swim according to the antidepressant treatments schedule. Behaviorally naïve rats not exposed to the forced swimming and antidepressant served as baseline controls (CON) for each set. All rats were sacrificed 1 h after the last antidepressant or saline injection. Abbreviations used: SAL, saline-treated rats not exposed to the pretest swim; DMI, desipramine-treated rats not exposed to the pretest swim; CIT, citalopram-treated rats not exposed to the pretest swim; pTS+SAL, pretest swim-exposed, saline-treated rats; pTS+DMI, pretest swim-exposed, desipramine-treated rats; pTS+CIT, pretest swim-exposed, citalopram-treated rats.

Changes in intentions regarding sun protection and screening (pretest-posttest). (a) Sunscreen use. (b) Shade seeking. For sunscreen use and shade-seeking outcomes, intend to improve is indicated by lower pretest utilization (never, sometimes) and higher posttest intentions (often or always). Intend to maintain is indicated by higher pretest utilization (often or always) and higher posttest intentions (often or always). No intention to improve is indicated by lower pretest utilization (never, sometimes) and lower posttest intentions (never, sometimes). Finally, intend to relapse is indicated by higher pretest utilization (often or always) and lower posttest intentions (never, sometimes). (c) Skin cancer screening by a healthcare professional. (d) Skin cancer self-screening. For screening outcomes, intend to improve is indicated by no reported prior screening at pretest but intentions to screen at posttest. Intend to maintain is indicated by reported prior screening at pretest and intentions to screen at posttest. No intention to improve is indicated by no reported prior screening at pretest and no intentions to screen at posttest. Intend to relapse are indicated by reported prior screening at pretest, yet no intentions to screen at posttest.

Variables present represent the significant predictors of pretest and/or change in Overall environmental literacy from the full models represented in and . Pretest scores represent total MSELS score at time of pretest and change in scores represents difference between pretest and post-test scores attributed to each variable independent of the others in the model.